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KMID : 0380020090240060556
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 6 p.556 ~ p.562
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Biological Treatment of TNT-containing Wastewater (pink water) by Stenotrophomonas maltophilia OK-5, and RT-PCR Quantification of the Nitroreductase ( pnrB) Gene
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Cho Su-Hee
Cho Yun-Seok
Oh Kye-Heon
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Abstract
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he biological treatment of TNT-containing wastewater, known commonly as pink water, was investigated using a stirred tank reactor with Stenotrophomonas maltophilia OK-5 bacterial culture. S. maltophilia OK-5 exhibited effective degradation of TNT contained in pink water, completely degrading TNT (100 mg/L) within 6 days of incubation. The dark-red brown color derived from Hydride-Meisenheimer complex became more pronounced during the incubation period, which was determined quantitatively. High-pressure liquid chromatography was used to measure residual TNT, which also resolved the metabolic intermediates (i.e., 2,4-dinitrotoluene, 2,6-dinitrotoluene and 2,4-dinitro-6-hydroxytoluene). Gas chromatography-mass spectrometry was used to verify these intermediates. Quantification of the nitroreductase (pnrB) gene isolated from S. maltophilia OK-5 growing in pink water was performed with real-time PCR. The amount of pnrB gene copies increased to 103-fold after 5 days of incubation time.
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KEYWORD
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Pink water, 2, 4, 6-trinitrotoluene (TNT), Biodegradation, Stenotrophomonas maltophilia OK-5, Real-time PCR
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